4E: Nanomaterials (SIG)

Timeslot: Thursday, April 20, 2023 - 1:30pm to 3:30pm
Room: Coral 3-4

About

In this study, we show the effect of various LED wavelengths (blue, green, red and near infrared (NIR)) as physical regulators for gene delivery to stem cells by promoting nanomaterials behavior and regulating cell membrane permeability.
The size of nanoparticles produced with various LED wavelengths was evaluated in the colloidal state (agglomeration to single form). And physical stimulation of cell membranes using two type of LED wavelengths (blue and NIR) enhanced penetration of extracellular substances. The regulation of nanoparticles size and cell membrane permeability effectively increased the gene delivery to cells and protein expression by accelerating transfection process (cellular uptake, endosomal escape, PEI-gene dissociation).
LED effects on nanoparticles was confirmed using DLS, SEM, and Nanosight. In addition, cell membrane permeability was assessed by confocal laser microscopy, FACS and then transfection efficiency was demonstrated using confocal laser microscopy, FACS and Western blot.
Reduction of nanoparticles size by LED have potential value for stem cell therapy as LED-induced gene delivery system. Also, by increasing the cell membrane permeability, extracellular substances that cannot easily penetrate the cell membrane can be delivered effectively.

  • 1:30 PM. 165. Ionizable Lipid Nanoparticle Platform for in Vivo Delivery of Small Protein Scaffolds for Potent RAS Inhibition. Rebecca Haley, Alexander Chan, Margaret Billingsley, Andrew Tsourkas, Michael Mitchell, PhD, University of Pennsylvania.

  • 1:45 PM 166. Bone Marrow Vascular Microenvironment Combination RNAi Nanomaterials Therapy for Multiple Myeloma. Christian Figueroa-Espada, Pedro P. G. Guimarães, PhD, Michael J. Mitchell, PhD, University of Pennsylvania, Universidade Federal de Minas Gerais.

  • 2:00 PM 167. Targeting of Porous Silicon Nanoparticles to the Traumatically Injured Brain for the Delivery of Growth Factors. Jonathan M. Zuidema, PhD, Lauren E. Waggoner, Jinyoung Kang, PhD, Sanahan Vijayakumar, PhD, Alan A. Hurtado, Michael J. Sailor, PhD, Ester J. Kwon, PhD, University of California San Diego, Mario Negri Institute for Pharmacological Research.

  • 2:15 PM 168. Use of a Keap1-inhibiting Peptide Brush Polymer for Myocardial Infarction Treatment. Joshua M. Mesfin, Kendal P. Carrow, Alexander Chen, Emma Zelus, Jervaughn Hunter, Elyse G. Wong, Colin G. Luo, Nathan C. Gianneschi, Karen L. Christman, UC San Diego, Sanford Consortium for Regenerative Medicine, Northwestern University.

  • 2:30 PM 169. Characterizing NP Fate in the CNS After Intrathecal Administration: A PET/CT Imaging Approach. Rachael Sirianni, Oluwatobi Babayemi, Corinne Fotso, Fred Christian Velasquez, Sauradip Chaudhuri, Lindsey Sablatura, Janelle Morton, Eva Sevick-Muraca, Rice University.

  • 2:45 Pm 170. Reprogramming Clots for In Vivo Chemical Targeting of Nanomaterials in Traumatic Brain Injury. Rebecca Kandell, Jason Wu, Ester J. Kwon, PhD, UC San Diego.

  • 3:00 PM 171.Nanoformulation of a Peptide Prophylactic for the Treatment of Traumatic Brain Injury. Jason Wu, Akash Canjels, Ester J. Kwon, University of California-San Diego.

  • 3:15 PM 172. Development of Lipid-Polymer Hybrid Nanoparticles for the Co-Encapsulation of 6-Bromo-Indirubin-3’-Oxime and Copper Diethyldithiocarbamate for Synergistic Cancer Therapy. Radu Paun, Daciana C. Dumut, Danuta Radzioch, Maryam Tabrizian, Faculty of Medicine and Health Sciences, McGill University, Research Institute of the McGill University Health Centre, Faculty of Dental Medicine and Oral Health Sciences, McGill Universit.